This Human MAD2L1-binding protein (MAD2L1BP) ELISA Kit employs a two-site sandwich ELISA to quantitate MAD2L1BP in samples. An antibody specific for MAD2L1BP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAD2L1BP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAD2L1BP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAD2L1BP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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