Human Klotho (KL) ELISA Kit employs a two-site sandwich ELISA to quantitate Klotho in samples. An antibody specific for Klotho has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Klotho present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human Klotho detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Klotho bound in the initial step. The color development is stopped and the intensity of the color is measured.
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