Human Anti-Rotavirus IgG ELISA Kit quantitate Human Anti-Rotavirus IgG in samples. An antigen has been pre-coated onto a microplate. Standards or test samples are added to the wells, incubated and then washed. HRP-conjugated anti-human IgG antibody is then added and incubated. The plate is washed once more and the chromogen solution is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.
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