EliKine(TM) Mouse IL-1alpha ELISA Kit

Artikelnummer: ASC-KTE7004
Artikelname: EliKine(TM) Mouse IL-1alpha ELISA Kit
Artikelnummer: ASC-KTE7004
Hersteller Artikelnummer: KTE7004
Alternativnummer: ASC-KTE7004-48, ASC-KTE7004-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Mouse
Alternative Synonym: Hematopoietin 1, IL 1 alpha, Il 1a, IL1, IL1 ALPHA, IL1A, IL1F1, Ilia, Interleukin 1 alpha, Interleukin 1 alpha precursor, Interleukin1 alpha
EliKine(TM) Mouse IL-1alpha ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-1alpha.
Detektionsbereich: 7.82 pg/mL-500 pg/mL
Sensitivitaet: 4 pg/mL
Tag: IL1A
NCBI: 16175
UniProt: P01582
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: EliKine(TM) Mouse IL-1alpha ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1alpha in samples. An antibody specific for IL-1alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1alpha is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.