EliKine(TM) Mouse VEGF ELISA Kit

Artikelnummer: ASC-KTE7016
Artikelname: EliKine(TM) Mouse VEGF ELISA Kit
Artikelnummer: ASC-KTE7016
Hersteller Artikelnummer: KTE7016
Alternativnummer: ASC-KTE7016-48,ASC-KTE7016-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Mouse
Alternative Synonym: VEGFA, RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165, vascular permeability factor
EliKine(TM) Mouse VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse VEGF-A.
Detektionsbereich: 15.63 pg/ml-1000 pg/ml
Sensitivitaet: 8 pg/ml
Tag: VEGFA
NCBI: 22339
UniProt: Q00731
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: EliKine(TM) Mouse VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF-A in samples. An antibody specific for VEGF-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF-A is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound in the initial step. The color development is stopped and the intensity of the color is measured.