This Mouse Protein kish-B (TMEM167B) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM167B in samples. An antibody specific for TMEM167B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM167B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM167B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM167B bound in the initial step. The color development is stopped and the intensity of the color is measured.
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