EliKine(TM) Mouse GLP-1 Competitive ELISA Kit uses a competitive method to quantify mouse GLP-1 in a sample. Mouse specific antibody against glucagon-like peptide 1(GLP-1) was coated on the enzyme label plate, and the antigen to be tested (sample or standard) and biotin-labeled antigen were added to the well of the enzyme label plate at the same time. The antigen to be tested and the biotin-labeled antigen were competitively bound to the specific antibody. After washing, the proprietary streptavidin-HRP conjugate was added to the wells. After washing and removing any unbound streptavidin-HRP, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Mouse GLP-1 concentration is inversely proportional to the OD450 nm value.
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