This Mouse Motilin (MTL) ELISA Kit employs a two-site sandwich ELISA to quantitate MTL in samples. An antibody specific for MTL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTL bound in the initial step. The color development is stopped and the intensity of the color is measured.
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