Recombinant Mouse Monoclonal Anti-PLA2R1 Antibody against Human phospholipase A2 receptor 1, 180kDa. Validated for Immunofluorescence, Immunohistochemistry and Western Blot
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemical staining of human kidney shows weak membranous positivity in cells in glomeruli.
Validation of the Anti-PLA2R1 monoclonal antibody by comparing the immunofluorescence staining in human cell lines Hela and MCF7 exhibiting a relatively high and low expression of PLA2R1 (based on RNA-seq values), respectively. The PLA2R1 (green signal) is present in Hela cells but is absent in MCF7 cells. The anti-HDAC1 antibody (HPA029693) was used as a loading control (red signal).
Immunohistochemistry analysis in human kidney and pancreas tissues using AMAb90772 antibody. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.
Western blot analysis in human kidney tissue.
Immunohistochemical staining of human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.
Immunofluorescence staining of HeLa cells using the Anti-PLA2R1 monoclonal antibody, showing specific staining in the cytosol and plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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