E.coli-derived human AATF recombinant protein (Position: Q5-H552). Human AATF shares 78.5% and 79.9% amino acid (aa) sequence identity with mouse and rat AATF, respectively.
Alternative Synonym:
AATF, CHE1, DED, HSPC277, Protein AATF, Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1
Boster Bio Anti-AATF Antibody Picoband catalog A03945-3. Tested in WB, IHC, ICC/IF, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Klonalität:
Polyclonal
Konzentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Western blot, 0.25-0.5 µg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 1-2 µg/ml, Human, Mouse Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Immunofluorescence, 5 µg/ml, Rat ELISA, 0.1-0.5 µg/ml, -
IHC analysis of AATF using anti-AATF antibody (A03945-3). AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-AATF Antibody (A03945-3) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of AATF using anti-AATF antibody (A03945-3). AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-AATF Antibody (A03945-3) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of AATF using anti-AATF antibody (A03945-3). AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-AATF Antibody (A03945-3) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of AATF using anti-AATF antibody (A03945-3). AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-AATF Antibody (A03945-3) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
Western blot analysis of AATF using anti-AATF antibody (A03945-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AATF antigen affinity purified polyclonal antibody (Catalog A03945-3) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for AATF at approximately 95 kDa. The expected band size for AATF is at 63 kDa.
IF analysis of AATF using anti-AATF antibody (A03945-3) and anti-Beta Tubulin antibody (M01857-3).AATF was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
