E.coli-derived human gamma Catenin recombinant protein (Position: M556-A745). Human gamma Catenin shares 98% amino acid (aa) sequence identity with both mouse and rat gamma Catenin.
Boster Bio Anti-gamma Catenin/JUP Antibody Picoband catalog PB9185. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Klonalität:
Polyclonal
Konzentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugatio
Reinheit:
Immunogen affinity purified.
Formulierung:
Lyophilized
Target-Kategorie:
Junction plakoglobin
Application Verdünnung:
Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Rat Immunocytochemistry/Immunofluorescence, 2µg/ml, Human Flow Cytometry (Fixe
IHC analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). gamma Catenin was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-gamma Catenin Antibody (PB9185) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). gamma Catenin was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-gamma Catenin Antibody (PB9185) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). gamma Catenin was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-gamma Catenin Antibody (PB9185) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). gamma Catenin was detected in frozen section of rat intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-gamma Catenin Antibody (PB9185) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
ICC analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). gamma Catenin was detected in immunocytochemical section of human MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mi
Western blot analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates, Lane 4: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-gamma Catenin antigen affinity purified polyclonal antibody (Catalog PB9185) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for gamma Catenin at approximately 82 kDa. The expected band size for gamma Catenin is at 82 kDa.
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