The TNF-alpha Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the TNF-alpha promoter. Tumor necrosis factor-alpha (TNF-alpha) is one of the major proinflammatory cytokines and can induce systemic inflammation, apoptotic cell death, sepsis and cachexia. Dysregulation of TNF-alpha induction is often involved in various human diseases including inflammatory bowel disease, cancer and Alzheimers disease. The TNF-alpha induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1.
Puffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Verdünnung:
<h2>Application:</h2>Monitor the TNF-alpha induction activity.Screen for activators or inhibitors of the TNF-alpha signaling pathway. <h2>Culture conditions:</h2> Cells should be grown at 37C with 5% CO2 using DMEM medium supplemen
Fig-1: Induction of TNF-alpha promoter activity by phorbol 12-myristate 13-acetate in TNF-alpha HEK293 cells.
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