The TLR3/IFNB Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses full-length human Toll-like receptor 3 (TLR3) and Renilla luciferase reporter gene under the transcriptional control of the human IFN-beta (IFNB) promoter. Functional activity of the cell line has been validated by TLR3 ligand assay (Figure 1), in which upon activation by poly (I:C), TLR3 quickly initiates TRIF-dependent signaling pathway and mediates nuclear translocation of IRF3 that induces IFNB promoter activity.The TLR3/IFNB activation by poly (I:C) is shown in Figure 1.
Puffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Verdünnung:
<h2>Application:</h2>Monitor the TLR3 signaling pathway activity.Screen for activators or inhibitors of the TLR3 signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medium supplemented
Fig-1: Induction of TLR3 activity by poly(I:C) in TLR3/IFNBr HEK293 cells.
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