The SRE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the serum response element (SRE). The SRE reporter cell line is designed to monitor MAPK/ERK activity and can be used for studying GPCR-linked MAPK/ERK signaling pathways as well as screening of agonists, antagonists or signaling inhibitors related with the MAPK/ERK signaling pathways. Functional activity of the cell line has been validated by serum stimulation assay (Figure 1).
Puffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Verdünnung:
<h2>Application:</h2> Monitor the GPCR-linked MAPK/ERK signaling pathway. Screen for activators or inhibitors of the GPCR-linked MAPK/ERK signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using
Fig-1: Induction of SRE activity by FBS or FBS plus PMA in SRE HEK293 cells.
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