The ATF6 Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the activating transcription factor 6 (ATF6)-response element. ATF6 is a member of the basic-leucine zipper transcription factor family, which is located in the endoplasmic reticulum (ER) membranes and plays a central role in transcriptional activation of ER molecules. The ATF6 induction by Tunicamycin is shown in Figure 1.
Puffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Verdünnung:
<h2>Application:</h2> Monitor ATF6 transcriptional activity. Screen for activators or inhibitors of the ATF6 signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medium supplemented wit
Fig-1: Induction of ATF6 activity by Tunicamycin in ATF6 HeLa cells.
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