The TLR5/NF-kB Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses full-length human Toll-like receptor 5 (TLR5) and Renilla luciferase reporter gene under the transcriptional control of the NF-kB response element. TLR5 is one of the key innate immune receptors. Functional activity of the cell line has been validated by TLR5 ligand assay, in which upon activation by Flagellin, TLR5 quickly initiates downstream signaling pathway and mediates nuclear translocation of NF-kB (Figure 1).
Puffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Verdünnung:
<h2>Application:</h2>Monitor the TLR5 signaling pathway activity.Screen for activators or inhibitors of the TLR5 signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medium supplemented
Fig-1: Induction of TLR5 activity by Flagellin in TLR5/NF-kB HEK293 cells.
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