Fluorescent conjuates of alpha-Bungarotoxin bind to the nicotinic acetylcholine receptor with high affinity, and thus can be used to image the distribution of the receptor at the neuromuscular junctions. CF dyes are Biotiums line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF594 has excitation/emission at 593/614 nm.
Quelle:
Animal
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