Lumitein(TM) is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels, but that can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. It is as sensitive as the best silver stain by detecting 1 ng or less protein. Unlike silver stain, however, Lumitein(TM) has a linear detection range of at least 3 orders of magnitude. Gels can be stained in 90 minutes or less without a separate fixation step. Lumitein(TM) has an excitation spectrum that makes detection possible with either a simple UV box or fluorescence laser scanner. Moreover, protein gel staining with Lumitein(TM) is compatible with downstream protein analyses such as mass spectrometry and Edman-based sequencing.
Quelle:
Synthetic
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