Human IgM Immunoglobulin(IM373), CF405S conjugate, 0.1mg/mL, Clone: [IM373], Mouse, Monoclonal

Produktübersicht

• Artikelname: Human IgM Immunoglobulin(IM373), CF405S conjugate, 0.1mg/mL, Clone: [IM373], Mouse, Monoclonal
• Artikelnummer: BOT-BNC040373-500
• Hersteller Artikelnummer: BNC040373-500
• Alternativnummer: BOT-BNC040373-500-500UL
• Hersteller: Biotium
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant full-length heavy chain of human IgM
• Konjugation: CF405S
• Alternative Synonym: AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region

Produktbeschreibung

Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.1 mg/mL
• Klon-Bezeichnung: [IM373]
• Molekulargewicht: 50-75 kDa
• UniProt: P01871
• Puffer: PBS, 0.1% BSA, 0.05% azide
• Quelle: Animal

Anwendungsbeschreibung

• Anwendungsbeschreibung: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user