This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Klonalität:
Monoclonal
Konzentration:
0.1 mg/mL
Klon-Bezeichnung:
[Hyb8]
Molekulargewicht:
244Da
Isotyp:
IgG1
UniProt:
Not Applicable
Puffer:
PBS, 0.1% BSA, 0.05% azide
Quelle:
Animal
Anwendungsbeschreibung:
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|No special pretreatment is required for the immunohistochemical staining of formalin-fixed paraffin-embedded tissues.|Immunohistology formalin-fixed 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting
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