Blood Group Antigen A (CD173)(3-3A), CF405S conjugate, 0.1mg/mL, Clone: [3-3A], Mouse, Monoclonal
Artikelnummer:
BOT-BNC041251-100
Hersteller Artikelnummer:
BNC041251-100
Alternativnummer:
BOT-BNC041251-100-100UL
Hersteller:
Biotium
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
IHC
Spezies Reaktivität:
Human
Immunogen:
Mucin isolated from an ovarian cyst fluid
Konjugation:
CF405S
Alternative Synonym:
ABO Type A, Blood Group A
This antibody is applicable for staining ABO blood groups A and AB. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens. This antibody preferentially reacts with determinants of chain A and H type 3 (Gal1-3GalNAc-R) and 4 (Gal1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistochemistry (formalin-fixed): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user
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