Ki-67 (Proliferating Cell Marker) (MKI67/2462), CF405S conjugate, 0.1mg/mL, Clone: [MKI67/2462], Mouse, Monoclonal

Produktübersicht

• Artikelname: Ki-67 (Proliferating Cell Marker) (MKI67/2462), CF405S conjugate, 0.1mg/mL, Clone: [MKI67/2462], Mouse, Monoclonal
• Artikelnummer: BOT-BNC042462-100
• Hersteller Artikelnummer: BNC042462-100
• Alternativnummer: BOT-BNC042462-100-100UL
• Hersteller: Biotium
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant human Ki67 protein fragment (around aa 2293-2478) (exact sequence is proprietary)
• Konjugation: CF405S
• Alternative Synonym: KI-67, Ki67, KI-67 Antigen (KIA), MKI67, Proliferation related Ki-67 antigen

Produktbeschreibung

Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki-67 index and the histo-pathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki-67 is a good marker of proliferating cell populations. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.1 mg/mL
• Klon-Bezeichnung: [MKI67/2462]
• Molekulargewicht: 345 kDa and 395 kDa
• UniProt: P46013
• Puffer: PBS, 0.1% BSA, 0.05% azide
• Quelle: Animal

Anwendungsbeschreibung

• Anwendungsbeschreibung: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user