p53(DO-7), CF640R conjugate, 0.1mg/mL, Clone: [DO-7], Mouse, Monoclonal

Produktübersicht

• Artikelname: p53(DO-7), CF640R conjugate, 0.1mg/mL, Clone: [DO-7], Mouse, Monoclonal
• Artikelnummer: BOT-BNC400513-100
• Hersteller Artikelnummer: BNC400513-100
• Alternativnummer: BOT-BNC400513-100-100UL
• Hersteller: Biotium
• Wirt: Mouse
• Kategorie: Antikörper
• Spezies Reaktivität: Bovine, Human, Monkey
• Immunogen: Recombinant human wild type p53 protein expressed in E. coli.
• Konjugation: CF640R
• Alternative Synonym: Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53

Produktbeschreibung

Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (aa 37-45) of p53. Monoclonal antibody PAb1801 does not block the binding of DO-7 MAb to p53 in an ELISA test. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.1 mg/mL
• Klon-Bezeichnung: [DO-7]
• Molekulargewicht: 53 kDa
• UniProt: P04637
• Puffer: PBS, 0.1% BSA, 0.05% azide
• Quelle: Animal

Anwendungsbeschreibung

• Anwendungsbeschreibung: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry