NKX6.1 (Marker for Pancreatic and Duodenal Neuroendocrine Tumors) (NKX61/2561), CF740 conjugate, 0.1mg/mL, Clone: [NKX61/2561], CF 740, Mouse, Monoclonal
Artikelnummer:
BOT-BNC742561-100
Hersteller Artikelnummer:
BNC742561-100
Alternativnummer:
BOT-BNC742561-100-100UL
Hersteller:
Biotium
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
IHC
Spezies Reaktivität:
Human
Immunogen:
Human full-length recombinant NKX6.1 protein
Konjugation:
CF 740
Alternative Synonym:
Homeobox protein NK-6 homolog A, Homeobox protein Nkx-6.1, NK homeo box, family 6, member A, NK homeobox (Drosophila), family 6, A, NK homeobox, family 6, A, NK6 homeobox 1, NK6 transcription factor homolog A, NK6 transcription factor related, locus 1, NK6 transcription factor related, locus 1 (Drosophila), NKX6-1, NKX6.1, NKX61, NKX61_HUMAN, NKX6A
Members of the Nkx family of homeodomain proteins are key regulators of growth and development in several tissues, including brain, heart and pancreas. During neural development, sonic hedgehog (Shh) is known to control cell fate and mitogenesis, which is correlated with Shh dose-dependent expression of several genes, including Nkx-6.1. Specifically, Nkx-6.1 is responsible for cellular differentiation in the ventral neural tube and spinal meninges in response to Shh. In the pancreas, Nkx-6.1 is exclusively expressed in the islets of Langerhans in differentiating and mature B cells, which produce Insulin. The presence of Pdx-1 is required for the expression of Nkx-6.1 as well as other pancreatic B cell specific genes, including Insulin, Glut2 and IAPP. Subsequently, Nkx-6.1 binds to the DNA consensus sequence, TTAATTAC, to direct the repression of specific genes in B cells.Nkx6.1 is highly expressed in pancreatic and duodenal well-differentiated neuroendocrine tumors (WDNETS) and in metastatic WDNETS, is a highly specific marker of tumors of pancreatic origin. It has thus been suggested that Nkx6.1 is a useful inclusion into IHC panels for identifying primary sites of WDNETS.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user
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