AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2552), Biotin conjugate, 0.1mg/mL

Produktübersicht

• Artikelname: AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2552), Biotin conjugate, 0.1mg/mL
• Artikelnummer: BOT-BNCB2552-500
• Hersteller Artikelnummer: BNCB2552-500
• Alternativnummer: BOT-BNCB2552-500-500UL
• Hersteller: Biotium
• Kategorie: Antikörper
• Applikation: IHC-P, WB
• Konjugation: Biotin

Produktbeschreibung

This antibody recognizes a protein of 62 kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBbeta or RacPK-beta) and Akt3 (also designated PKB-gamma or thymoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-beta tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Produkteigenschaften

• Konzentration: 0.1 mg/mL
• Puffer: PBS, 0.1% BSA, 0.05% azide
• Quelle: Animal