PHA stimulated human peripheral blood lymphocytes.
Alternative Synonym:
CD100, Semaphorin-4D, SEMA4D
CD100 (also known as Semaphorin 4D), is a homodimeric protein comprised of 50 kDa subunits. It is expressed on resting T cells and weakly on other lymphocytes, and its expression is increased on B and T cells after stimulation. CD100 is a cell surface receptor that binds to CD72, Plexin B1 and Plexin B2. It promotes B cell aggregation, cell survival, and cell proliferation.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Functional studies: Order Ab without azide, Flow cytometry: 0.5-1 ug/million cells, Immunofluorescence: 0.5-1 ug/mL, Optimal dilution for a specific application should be determined.
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