Major Vault Protein (MVP)(1032), 0.2mg/mL, Clone: [1032], Mouse, Monoclonal

Produktübersicht

• Artikelname: Major Vault Protein (MVP)(1032), 0.2mg/mL, Clone: [1032], Mouse, Monoclonal
• Artikelnummer: BOT-BNUB0225-100
• Hersteller Artikelnummer: BNUB0225-100
• Alternativnummer: BOT-BNUB0225-100-100UL
• Hersteller: Biotium
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Proteins precipitated from human breast cancer MCF-7 cells
• Alternative Synonym: Lung resistance-related protein (LRP), Major Vault Protein, MVP, VAULT1

Produktbeschreibung

Recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.2 mg/mL
• Klon-Bezeichnung: [1032]
• Molekulargewicht: 104-110 kDa
• UniProt: Q14764
• Puffer: PBS, 0.05% BSA, 0.05% azide
• Quelle: Animal

Anwendungsbeschreibung

• Anwendungsbeschreibung: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user