Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Klonalität:
Monoclonal
Konzentration:
0.2 mg/mL
Klon-Bezeichnung:
[PY20]
Molekulargewicht:
Depends upon the phosphorylated target
Isotyp:
IgG2b
UniProt:
Not Applicable
Puffer:
PBS, 0.05% BSA, 0.05% azide
Quelle:
Animal
Anwendungsbeschreibung:
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 1-2 ug/mL|No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Immunoprecipitation 1-2 ug/500 ug protein lysate|Optimal dilution for a specific application should be determined by user
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