Lewis A(7LE), 0.2mg/mL, Clone: [7LE], Mouse, Monoclonal

Produktübersicht

• Artikelname: Lewis A(7LE), 0.2mg/mL, Clone: [7LE], Mouse, Monoclonal
• Artikelnummer: BOT-BNUB0311-100
• Hersteller Artikelnummer: BNUB0311-100
• Alternativnummer: BOT-BNUB0311-100-100UL
• Hersteller: Biotium
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, FC, IHC
• Spezies Reaktivität: Human, Mouse
• Immunogen: Mucins isolated from ovarian cyst fluid
• Alternative Synonym: Lewis A antigen

Produktbeschreibung

Recognizes a carbohydrate determinant of Gal 1-3(Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.2 mg/mL
• Klon-Bezeichnung: [7LE]
• Molekulargewicht: Multiple
• UniProt: Not Applicable
• Puffer: PBS, 0.05% BSA, 0.05% azide
• Quelle: Animal

Anwendungsbeschreibung

• Anwendungsbeschreibung: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin)|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user