BrdU (Bromodeoxyuridine)(BRD469 + BRD494 + BRD.3), 0.2mg/mL, IgG1, Clone: [BRD469 BRD494 BRD.3], Mouse, Monoclonal

Artikelnummer: BOT-BNUB1000-100
Artikelname: BrdU (Bromodeoxyuridine)(BRD469 + BRD494 + BRD.3), 0.2mg/mL, IgG1, Clone: [BRD469 BRD494 BRD.3], Mouse, Monoclonal
Artikelnummer: BOT-BNUB1000-100
Hersteller Artikelnummer: BNUB1000-100
Alternativnummer: BOT-BNUB1000-100-100UL
Hersteller: Biotium
Wirt: Mouse
Kategorie: Antikörper
Applikation: IHC
Spezies Reaktivität: All
Immunogen: Bromodeoxyuridine (BrdU) conjugated to BSA or KLH
Alternative Synonym: Bromodeoxyuridine, 5-bromo-2-deoxyuridine
Bromodeoxyuridine (BrdU) is a synthetic nucleoside thymidine analog. It is commonly used to detect proliferation in cells. In cells cultured in the presence of BrdU, BrdU will incorporate into newly synthesized DNA during S-phase. Antibodies specific for BrdU can then be used to detect replicated DNA. This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL).
Klonalität: Monoclonal
Konzentration: 0.2 mg/mL
Klon-Bezeichnung: [BRD469 BRD494 BRD.3]
Molekulargewicht: Depends on the target
Isotyp: IgG1
UniProt: Not Applicable
Puffer: PBS, 0.05% BSA, 0.05% azide
Quelle: Animal
Anwendungsbeschreibung: Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/mL PBS, 10 min at 37C|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells|Optimal dilution for a specific application should be determined by user