DNA methyltransferase 3 alpha, DNA methyltransferase HsaIIIA, DNAMTaseHsaIIIA, DNMT, DNMT3A2, M.HsaIIIA, MCMT, TBRS
The specificity of this monoclonal antibody to its intended target was validated by HuProt(TM)Array, containing more than 19,000, full-length human proteins. DNMT3A is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated Lys-36 of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Immunoprecipitation 2-4 ug/mg protein|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes|Western blotting 0.5-1.0 ug/mL|Optimal dilution for a specific application should be determined by user
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