DNA Ligase 1(1A9), 1mg/mL, Clone: [1A9], Mouse, Monoclonal
Artikelnummer:
BOT-BNUM2177-50
Hersteller Artikelnummer:
BNUM2177-50
Alternativnummer:
BOT-BNUM2177-50-50UL
Hersteller:
Biotium
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
FC, Functional Studies, IP
Spezies Reaktivität:
Bovine, Human, Mouse, Rat
Immunogen:
Full-length native cow LIG1 protein
Alternative Synonym:
DNA ligase 1, DNA ligase I, DNLI1_HUMAN, LIG 1, lig1, Ligase I DNA ATP dependent, MGC117397, MGC130025, Polydeoxyribonucleotide synthase [ATP] 1
DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5 phosphoryl and 3 hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. DNA Ligase I activity is altered in the chromosomal breakage deficit Blooms syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
