VISTA / GI24 (Negative Regulator of Immune Response)(VISTA/2864), 1mg/mL, Clone: [VISTA/2864], Mouse, Monoclonal
Artikelnummer:
BOT-BNUM2864-50
Hersteller Artikelnummer:
BNUM2864-50
Alternativnummer:
BOT-BNUM2864-50-50UL
Hersteller:
Biotium
Wirt:
Mouse
Kategorie:
Antikörper
Spezies Reaktivität:
Human
Immunogen:
Recombinant full-length human VISTA protein
Alternative Synonym:
B7H5, DD1alpha, GI24, PD-1H, PDCD1 homolog, Platelet receptor Gi24, PP2135, Stress-induced secreted protein 1 (SISP1), V domain Ig suppressor of T cell activation, V Set Immuno-regulatory Receptor
VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC s, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
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