Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody. Major areas ofapplication include monitoring protein expression, localizing proteins at thecellular and subcellular levels, and protein purification, as well as the analysis of protein topology, dynamics and interactions. Due to their small size, theyare unlikely to affect the tagged proteins biochemical properties.S1 tag is an epitope tag composed of a nine-residue peptide, NANNPDWDF, derived from the hepatitis B virus preS1 region. Epitope tags consisting ofshort sequences recognized by well-characterizated antibodies have beenwidely used in the study of protein expression in various systems.
Klonalität:
Polyclonal
Konzentration:
1ug/ul
Puffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Quelle:
KLH conjugated synthetic peptide S1-Tag
Target-Kategorie:
S1-Tag
Application Verdünnung:
WB(1:300-5000), ELISA(1:500-1000)
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