Recombinant CRISPR-Cas9 protein, C-His, Unconjugated

Produktübersicht

• Artikelname: Recombinant CRISPR-Cas9 protein, C-His, Unconjugated
• Artikelnummer: BSS-BS-41498P
• Hersteller Artikelnummer: bs-41498P
• Alternativnummer: BSS-BS-41498P-20,BSS-BS-41498P-100,BSS-BS-41498P-500
• Hersteller: Bioss
• Kategorie: Proteine/Peptide
• Spezies Reaktivität: Other
• Konjugation: Unconjugated

Produktbeschreibung

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3-5 exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Produkteigenschaften

• Tag: C-His
• NCBI: 901176
• UniProt: Q99ZW2
• Puffer: 0.01M TBS(pH7.4)
• Quelle: Recombinant CRISPR-Cas9 protein is expressed in E.coli with C-His. It contains the amino acid sequence of 2-1368/1368
• Expression System: E.coli
• Reinheit: > 90% as determined by SDS- PAGE