The detection system facilitates the identification of mouse IgG antibody bond to an antigen in tissue sections. The specific antibody is pinpointed by a secondary antibody that is conjugated with a horseradish peroxidase (HRP) polymer, designed to specifically recognize mouse immunoglobulins. The polymer attached complex is subsequently made visible under light microscope using HRP-compatible chromogens, such as diaminobenzidine (DAB). The provided HRP polymer conjugated secondary antibody significantly address the limitations commonly experienced with traditional immunohistochemistry (IHC) methods, such as poor or inconsistent antigen staining when identifying low-abundance antigens or in situations of suboptimal antibody-antigen binding. By utilizing an HRP polymer conjugate, the sensitivity of detection is dramatically increased and the process is simplified. Moreover, the HRP polymer-based amplification method reduces the amount of primary antibody needed and shortens the secondary antibodies incubation period.
Target-Kategorie:
IgG H&L
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