ERK1/2 (Y204) polyclonal antibody, Unconjugated, Rabbit

Produktübersicht

• Artikelname: ERK1/2 (Y204) polyclonal antibody, Unconjugated, Rabbit
• Artikelnummer: BWT-AP0722
• Hersteller Artikelnummer: AP0722
• Alternativnummer: BWT-AP0722-50UL,BWT-AP0722-100UL
• Hersteller: Bioworld Technology
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Synthetic peptide, corresponding to Human ERK1/2.
• Konjugation: Unconjugated
• Alternative Synonym: Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, MAPK1, ERK2, PRKM1, PRKM2

Produktbeschreibung

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.

Produkteigenschaften

• Molekulargewicht: ~ 42, 44 kDa
• UniProt: P27361
• Reinheit: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Formulierung: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Anwendungsbeschreibung

• Application Verdünnung: WB: 1:500~1:1000 IP: 1:50~1:200 IF: 1:100~1:500
• Anwendungsbeschreibung: ERK1/2 (Y204) polyclonal antibody detects endogenous levels of ERK1/2 protein.