Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid. While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKalpha/beta, phosphorylation of IkappaBalpha at Ser32/36, and NF-kappaB activation. Finally, activation of the transcription factor NF-kappaB is responsible for the induction of Cox2 expression. Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock. Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimers disease by potentiating gamma-secretase activity and beta-amyloid generation.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Formulierung:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Verdünnung:
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
Anwendungsbeschreibung:
PTGS1 (S585) polyclonal antibody detects endogenous levels of PTGS1 protein.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
