Synthetic peptide, corresponding to amino acids 51-100 of Human PCNA.
Konjugation:
Unconjugated
Alternative Synonym:
Proliferating cell nuclear antigen, Cyclin
The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication, and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli, however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms, one involved in ongoing DNA replication. which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Formulierung:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Verdünnung:
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
Anwendungsbeschreibung:
PCNA (I88) polyclonal antibody detects endogenous levels of PCNA protein.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
