Synthetic peptide, corresponding to amino acids 196-245 of Human NIFK.
Konjugation:
Unconjugated
Alternative Synonym:
MKI67 FHA domain-interacting nucleolar phosphoprotein, Nucleolar phosphoprotein Nopp34, Nucleolar protein interacting with the FHA domain of pKI-67, hNIFK, NIFK, MKI67IP, NOPP34
The structural proteins for the complex metalloenzyme nitrogenase include NIFK, NIFD and NIFH. These proteins are all necessary for archaeal and bacterial nitrogen fixation. The NIFK gene encodes the b subunit of the nitrogenase molybdenum-iron (MoFe) tetramer. NIFK localizes to the nucleolus where it interactes with the fork-head associated domain of the proliferation marker protein Ki-67 in a mitosis-specific and phosphorylation-dependent manner. NIFK is widely expressed in adult tissues, suggesting other functions in addition to its interaction with Ki-67, which is only expressed in proliferating cells.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Formulierung:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Verdünnung:
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Anwendungsbeschreibung:
NIFK (D229) polyclonal antibody detects endogenous levels of NIFK protein.
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