Synthetic peptide, corresponding to amino acids 190-240 of Human APEX1.
Konjugation:
Unconjugated
Alternative Synonym:
AP lyase, APE 1, APE, APE-1, APEN, APEX 1, APEX, APEX nuclease (multifunctional DNA repair enzyme) 1, Apex nuclease 1, Apex nuclease, APEX1, Apurinic endonuclease, Apurinic-apyrimidinic endonuclease 1, Apurinic/apyrimidinic (abasic) endonuclease, Apurinic/apyrimidinic endonuclease 1, Apurinic/apyrimidinic exonuclease, APX, BAP1, Deoxyribonuclease (apurinic or apyrimidinic), DNA (apurinic or apyrimidinic site) lyase, DNA-(apurinic or apyrimidinic site) lyase, mit
The role of transcription factors in the regulation of gene expression is well established. Although the activity of these factors can be regulated by phosphorylation, evidence has indicated regulation of DNA binding mediated by changes in reduction-oxidation (redox) status. Mutational analysis has identified a single conserved cysteine residue mapping within the DNA binding domains of Fos and Jun. Chemical oxidation or modification of this cysteine residue inhibits the DNA binding activity of Fos and Jun. A similar mode of regulation has been recently proposed for other nuclear transcription factors. Oxidation is reversible by these compounds or by a cellular redox/DNA repair protein identified originally as Ref-1 (redox factor 1). Ref-1 is identical to a previously characterized DNA repair enzyme designated HAP1, APE or APEX.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Formulierung:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Verdünnung:
WB: 1:500~1:1000 IHC: 1:50~1:200
Anwendungsbeschreibung:
APEX1 (R221) polyclonal antibody detects endogenous levels of APEX1 protein.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
