Synthetic peptide, corresponding to amino acids 630-680 of Human POLI.
Konjugation:
Unconjugated
Alternative Synonym:
DNA polymerase iota, Eta2, RAD30 homolog B, POLI, RAD30B
DNA polymerase activity is essential for replication, repair, recombination and mutagenesis. DNA polymerases can often bypass DNA lesions that block DNA replication, thereby allowing the replication of damaged DNA. One such DNA polymerase is the distributive enzyme DNA pol iota (pol iota), which is encoded by the POLI gene. POLI is located on human chromosome 18q21.2, a region often implicated in the etiology of many human cancers. At thymine templates, DNA Pol iota is highly error-prone when replicating undamaged DNA in that it favors the misincorporation of guanine over the correct nucleotide, adenosine. DNA Pol iota also promotes the replication of damaged DNA by misincorporating deoxynucleotides opposite DNA lesions. DNA Pol iota acts sequentially with DNA Pol zeta, which is essential for damageinduced mutagenesis, to complete the DNA lesion bypass. Therefore, replication involving DNA Pol iota is likely to be highly mutagenic.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Formulierung:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Verdünnung:
WB: 1:500~1:1000 IHC: 1:50~1:200
Anwendungsbeschreibung:
POLI polyclonal antibody detects endogenous levels of POLI protein.
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