The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as a oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, alpha, beta and gamma, comprise to form the Na+/K+-ATPase oligomer. The alpha subunit contains the binding sites for ATP and the cations, the glycosylated beta subunit ensures correct folding and membrane insertion of the alpha subunits. The small gamma subunit co-localizes with the alpha subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The beta subunit, but not the gamma subunit, is essential for normal activity of Na+/K+-ATPase.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Formulierung:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Application Verdünnung:
WB: 1:500~1:1000
Anwendungsbeschreibung:
p-ATP1A1(S23) polyclonal antibody detects endogenous levels of ATP1A1 protein only when phosphorylated at Ser23 .
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