Synthetic phosphopeptide derived from human C/EBP epsilon around the phosphorylation site of Threonine 74.
Konjugation:
Unconjugated
Alternative Synonym:
CCAAT/enhancer-binding protein epsilon, C/EBP epsilon, CEBPE, C/EBPepsilon
C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally-differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma, C/EBP delta and C/EBP epsilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Formulierung:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Verdünnung:
WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200
Anwendungsbeschreibung:
C/EBP epsilon (Phospho-T74) polyclonal antibody detects endogenous levels of C/EBP epsilon protein only when phosphorylated at Thr74.
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