KLH-conjugated synthetic peptide encompassing a sequence within human Estrogen Receptor alpha. The exact sequence is proprietary.
Konjugation:
Unconjugated
Alternative Synonym:
ESR, NR3A1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
Estrogen receptor alpha (ERalpha), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains . Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERalpha regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation at multiple sites provides an important mechanism to regulate ERalpha activity . Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERalpha activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 . Ser167 may be phosphorylated by p90RSK and Akt . According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients .
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Formulierung:
Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Application Verdünnung:
IHC (1/100 - 1/300)
Anwendungsbeschreibung:
Recognizes endogenous levels of Estrogen Receptor alpha protein.
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