Creatine Kinase MM Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: Creatine Kinase MM Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB100001
• Hersteller Artikelnummer: orb100001
• Alternativnummer: BYT-ORB100001-50,BYT-ORB100001-100,BYT-ORB100001-200
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Creatine Kinase MM (221-330/381aa)
• Konjugation: Unconjugated
• Alternative Synonym: CKM, CKMM, CK-MM, Creatine kinase M, Creatine kinase M chain, Creatine kinase M type, Creatine kinase M-type, Creatine kinase muscle, KCRM_HUMAN, M-CK, MCK, MS785, Muscle creatine kinase.

Produktbeschreibung

Creatine Kinase MM Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 43 kDa
• UniProt: P06732
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: CKM

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Bilder

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Creatine Kinase MM) Polyclonal Antibody, Unconjugated (orb100001) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Skeletal Muscle Lysates, Lane 2: Mouse Heart Lysates, Lane 3: Rat Skeletal Muscle Lysates, Lane 4: Rat Heart Lysates, Primary: Anti-Creatine Kinase MM (orb100001) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43kDa, Observed band size: 50kDa.

Tissue/cell: rat heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Creatine Kinase MM/KCRM Polyclonal Antibody, Unconjugated (orb100001) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Creatine Kinase MM) Polyclonal Antibody, Unconjugated (orb100001) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.