KLH conjugated synthetic peptide derived from human Desmocollin 2 (355-460/901aa)
Konjugation:
Unconjugated
Alternative Synonym:
ARVD11, CDHF2, DG2, DGII/III, DSC3, CDHF3, DSC, DSC1, DSC2, DSC4, HT-CP, DSC2_HUMAN, Cadherin family member 2, Desmocollin-3, Desmosomal glycoprotein II, Desmosomal glycoprotein III, DSC3_HUMAN, Cadherin family member 3, Desmocollin-4,
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:
Liquid
Target-Kategorie:
DSC2
Application Verdünnung:
Flow-Cyt=2ug/Test
Blank control: K562. Primary Antibody (green line): Rabbit Anti-Desmocollin 2 + 3 antibody (orb100766) Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 µg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
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