KLH conjugated synthetic peptide derived from human SMYD3 (101-200/428aa)
Konjugation:
Unconjugated
Alternative Synonym:
KMT3E, ZMYND1, ZNFN3A1, bA74P14.1, 2410008A19Rik, SMYD3_HUMAN, SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1, 2.1.1.354, SMYD3_MOUSE,
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:
Liquid
Target-Kategorie:
SMYD3
Application Verdünnung:
IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SMYD3) Polyclonal Antibody, Unconjugated (orb100887) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
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