APAF1 (NT) Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: APAF1 (NT) Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB10108
• Hersteller Artikelnummer: orb10108
• Alternativnummer: BYT-ORB10108-100,BYT-ORB10108-200,BYT-ORB10108-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Apaf-1 (13-80/1248aa)
• Konjugation: Unconjugated
• Alternative Synonym: APAF 1, APAF1, APAF-1, Apoptotic peptidase activating factor 1, Apoptotic protease activating factor, CED 4, CED4, KIAA0413, APAF_HUMAN, Apoptotic protease-activating factor 1.

Produktbeschreibung

APAF1 (NT) Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 137 kDa
• UniProt: O14727
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: APAF1

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/test

Bilder

Tissue/cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-APAF1 (NT) Polyclonal Antibody, Unconjugated (orb10108) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-APAF1 (NT) Polyclonal Antibody, Unconjugated (orb10108) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell:SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (APAF1 (NT)) polyclonal Antibody, Unconjugated (orb10108) 1:100, 90 minutes at 37C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/cell:SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (APAF1 (NT)) polyclonal Antibody, Unconjugated (orb10108) 1:100, 90 minutes at 37C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

U-937 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with APAF1 (NT) Antibody (orb10108) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (APAF1 (NT)) Polyclonal Antibody, Unconjugated (orb10108) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (APAF1 (NT)) Polyclonal Antibody, Unconjugated (orb10108) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Lung (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-APAF1 (NT) (orb10108) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 140 kD, Observed band size: 140 kD.