TNMD Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: TNMD Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB101154
• Hersteller Artikelnummer: orb101154
• Alternativnummer: BYT-ORB101154-100,BYT-ORB101154-200,BYT-ORB101154-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human TNMD (151-250/317aa)
• Konjugation: Unconjugated
• Alternative Synonym: BRICD4, BRICHOS domain containing 4, CHM1 LIKE, CHM1L, Chondromodulin I like, Chondromodulin I like protein, Chondromodulin-1-like protein, Chondromodulin-I-like protein, hChM1L, hTeM, Myodulin, TEM, Tendin, Tenomodulin, Tenomodulin protein, TNMD, TNMD_HUMAN, UNQ771/PRO1565.

Produktbeschreibung

TNMD Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 37 kDa
• UniProt: Q9H2S6
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: TNMD

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Bilder

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TNMD) Polyclonal Antibody, Unconjugated (orb101154) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TNMD) Polyclonal Antibody, Unconjugated (orb101154) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Muscle (Rat) Lysate at 40 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: Heart (Rat) Lysate at 40 ug, Lane 4: SW480 (Human) Cell Lysate at 30 ug, Primary: Anti-TNMD (orb101154) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 35 kD.

Sample: Lane 1: Skeletal muscle (Rat) Lysate at 40 ug, Lane 2: Skeletal muscle (Mouse) Lysate at 40 ug, Primary: Anti-TNMD (orb101154) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 37 kD.

Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TNMD/tenomodulin Polyclonal Antibody, Unconjugated (orb101154) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human mammary gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TNMD) Polyclonal Antibody, Unconjugated (orb101154) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TNMD) Polyclonal Antibody, Unconjugated (orb101154) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TNMD) Polyclonal Antibody, Unconjugated (orb101154) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.